Immunoassay Reagents and Kits

Kits, substrates, and reagents for a variety of immunoassay procedures, including ELISA, singleplex immunoassays, and multiplex immunoassays, that are ideal for the quantification of target antigens; substrates available in colorimetric, chemiluminescent, or chemifluorescent varieties.

We offer a variety of products for enzyme-linked immunosorbent assays (ELISA or EIA) including plates, reagents, and kits to help you detect and quantify peptides, proteins, antibodies, and hormones.

ELISA is an analytical method based on the highly specific interaction between an antigen and an antibody. For this assay, an antigen is attached to a solid surface, to which an antibody-enzyme complex is linked. When a substrate is added and incubated with the other materials, it produces a measurable product.

Life Science Research ELISA Kits (132,619)

Singleplex and Multiplex Bead Based Immunoassays (6,174)

ELISA Substrates and Reagents (2,341)

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346 – 360 of 138,521 results

346

Invitrogen™ Mouse TNF alpha ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Mouse
Conjugate	HRP
Interassay CV	5.7%
Intraassay CV	6.5%
Gene Symbol	Tnf
Total Assay Time	3 hr 30 min
Sample Volume	Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity	3.7 pg/mL
Storage Requirements	2-8°C
Assay Range	31.3-2000 pg/mL
For Use With (Application)	ELISA
Test Time	1 hr 20 min
Accession Number	P06804
Regulatory Status	RUO
Target	TNF alpha
Kit Contents	Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Sample Diluent,Assay Buffer concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Absorbance Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	21926
Sample Type	Plasma,Serum,Supernatant
Label or Dye	Biotin
Synonym	Cachectin, Tumor necrosis factor alpha, DIF

347

Invitrogen™ ProcartaPlex™ Human Th1/Th2/Th9/Th17 Cytokine Panel, 18plex

The ProcartaPlex Human Th1/Th2/Th9/Th17 Cytokine Panel 18plex enables exploration of the T helper response by analyzing 18 protein targets in a single well using Luminex xMAP technology.

Pricing & Availability

348

Invitrogen™ Mouse Granzyme B ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Mouse
Conjugate	HRP
Intraassay CV	3.5%
Total Assay Time	3 hr 30 min
Sample Volume	Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity	1.6 pg/mL
Assay Range	7.8-500 pg/mL
For Use With (Application)	ELISA
Test Time	1 hr 20 min
Regulatory Status	RUO
Target	Granzyme B
Kit Contents	Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Sample Diluent,Assay Buffer concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Absorbance Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	14939
Sample Type	Plasma,Serum,Supernatant
Synonym	Granzyme 2, Fragmentin 2, Human lymphocyte protein

349

Invitrogen™ ProcartaPlex™ Rat Cytokine & Chemokine Panel, 22plex

The ProcartaPlex Rat Cytokine & Chemokine Panel 22plex enables the study of immune function by analyzing 22 protein targets in a single well using Luminex xMAP technology.

Pricing & Availability

350

Invitrogen™ ProcartaPlex™ NHP Cytokine & Chemokine Panel, 30plex

The ProcartaPlex Non-Human Primate Cytokine & Chemokine Panel 30plex enables research of immune function by analyzing 30 protein targets in a single well using Luminex xMAP technology.

Pricing & Availability

351

Invitrogen™ Mouse TNF alpha Uncoated ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Mouse
Conjugate	HRP
Gene Symbol	Tnf
Total Assay Time	24 hr 30 min
Sample Volume	Lavage Fluid 100 μL, Plasma 100 μL, Serum 100 μL, Supernatant 100 μL, Tissue Homogenate 100 μL
Assay Sensitivity	15.6 pg/mL
Storage Requirements	2-8°C
Assay Range	15.6-1000 pg/mL
For Use With (Application)	ELISA
Test Time	1 hr 45 min
Accession Number	P06804
Regulatory Status	RUO
Target	TNF alpha
Kit Contents	Capture Antibody: Pre-titrated, purified antibody, Detection Antibody: Pre-titrated, biotin-conjugated antibody, Standard: Recombinant cytokine for generating standard curve and calibrating samples, ELISA/ELISPOT Coating Buffer Powder: This uncoated ELISA kit may contain ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 μM) or 10X PBS ELISA Coating Buffer (Dilute 1 part 10X Buffer into 9 parts dH20)., Assay Diluent: 5X concentrated, Detection enzyme: Pre-titrated Avidin-HRP, Substrate Solution: Tetramethylbenzidine (TMB) Substrate Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86),Purified antibody,Detection Antibody: Pre-titrated,Biotin-conjugated antibody,Standard: Recombinant cytokine for generating standard curve and calibrating samples,ELISA/ELISPOT Coating Buffer Powder: This uncoated ELISA kit may contain ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 μM) or 10X PBS ELISA Coating Buffer (Dilute 1 part 10X Buffer into 9 parts dH20).,Assay Diluent: 5X concentrated,Detection enzyme: Pre-titrated Avidin-HRP,Substrate Solution: Tetramethylbenzidine (TMB) Substrate Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
For Use With (Equipment)	Absorbance Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	21926
Sample Type	Lavage Fluid,Plasma,Serum,Supernatant,Tissue Homogenates
Label or Dye	Biotin
Synonym	Tumor Necrosis Factor alpha, tnf-a,tnf alpha

352

Invitrogen™ Human Estradiol Rapid ELISA Kit

ELISA

Pricing & Availability
Specifications

Shipping Condition	Wet or Dry Ice
Immunoassay Kit Format	Coated ELISA Kit
Target Species	Human
Conjugate	HRP
Interassay CV	<10%
Intraassay CV	<10%
Total Assay Time	1 hr 30 min
Assay Sensitivity	13.22 pg/mL
Storage Requirements	Refer to product documentation for component specific storage temperature.
Assay Range	31.25-2000 pg/mL
Test Time	15 min
Regulatory Status	RUO
Target	Estradiol
Kit Contents	Pre-coated 96 well plate, Standard, HRP Conjugate, Standard & Sample Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer,Standard,HRP Conjugate,Standard & Sample Diluent,HRP Conjugate Diluent,Wash Buffer,TMB Substrate,Stop Solution,Plate Sealer
Gene Alias	17-Beta-Estradiol; 17-beta-HSD17-Beta-Estradiol; 17-beta-HSD;
Product Type	ELISA
For Use With (Equipment)	Absorbance Microplate Reader
Sample Type	Plasma,Saliva,Serum,Urine
Label or Dye	HRP

353

Invitrogen™ ProcartaPlex™ Mouse Cytokine & Chemokine Panel 1, 26plex

The ProcartaPlex Mouse Cytokine & Chemokine Panel 1 26plex enables the exploration of immune function by analyzing 26 protein targets in a single well using Luminex xMAP technology.

Pricing & Availability

354

Invitrogen™ Human IL-21 Uncoated ELISA Kit

ELISA

Pricing & Availability
Specifications

Protein Aliases	IL-21, Za11
Immunoassay Kit Format	Uncoated ELISA Kit
Content And Storage	Capture Antibody: Pre-titrated, purified antibody Detection Antibody: Pre-titrated, biotin-conjugated antibody Standard: Recombinant protein for generating standard curve and calibrating samples Coating Buffer: 10X PBS ELISA Coating Buffer Assay Diluent: 5X Concentrated Detection Enzyme: Pre-titrated Avidin-HRP Substrate Solution: Tetramethylbenzidine (TMB) Solution Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards. 96-well Plates: Corning Costar flat-bottom plates (included with Cat. Nos. ending in -22 and -86)
Target Species	Human
Conjugate	HRP
Gene Symbol	IL21
Total Assay Time	24 hr 30 min
Assay Sensitivity	8 pg/mL
Storage Requirements	2-8°C
Assay Range	8-1000 pg/mL
Test Time	1 hr 45 min
Accession Number	Q9HBE4
Regulatory Status	RUO
Target	IL-21
Kit Contents	Capture Antibody: Pre-titrated, purified antibody, Detection Antibody: Pre-titrated, biotin-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, Coating Buffer: 10X PBS ELISA Coating Buffer, Assay Diluent: 5X Concentrated, Detection Enzyme: Pre-titrated Avidin-HRP, Substrate Solution: Tetramethylbenzidine (TMB) Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22 and -86),Purified antibody,Detection Antibody: Pre-titrated,Biotin-conjugated antibody,Standard: Recombinant protein for generating standard curve and calibrating samples,Coating Buffer: 10X PBS ELISA Coating Buffer,Assay Diluent: 5X Concentrated,Detection Enzyme: Pre-titrated Avidin-HRP,Substrate Solution: Tetramethylbenzidine (TMB) Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22 and -86)
Gene Alias	CVID11; CX2; il 21; IL21; IL-21; ILN; Interleukin; interleukin 21; Interleukin21; interleukin-21; interleukin-21 isoform; porcine interleukin-21; Za11CVID11; CX2; il 21; IL21; IL-21; ILN; Interleukin; interleukin 21; Interleukin21; interleukin-21; interleukin-21 isoform; porcine interleukin-21; Za11; IL-6
For Use With (Equipment)	Absorbance Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	59067
Sample Type	Plasma,Serum,Supernatant
Label or Dye	Biotin

355

Invitrogen™ Mouse Galectin-9 ELISA Kit

ELISA

Pricing & Availability
Specifications

Conjugate	HRP
Interassay CV	<12%
Intraassay CV	<10%
Gene Symbol	Lgals9
Total Assay Time	4 hr 45 min
Sample Volume	Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity	0.18 ng/mL
Storage Requirements	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Assay Range	0.18-40 ng/mL
Test Time	1 hr 20 min
Accession Number	O08573
Regulatory Status	RUO
Target	Galectin 9
Kit Contents	Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Absorbance Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	16859
Sample Type	Plasma,Serum,Supernatant
Label or Dye	Biotin

356

Invitrogen™ IkB alpha Recombinant Rat Monoclonal Antibody (MFRDTRK), Alexa Fluor™ Plus 488

Rat Recombinant Monoclonal Antibody

Pricing & Availability
Specifications

Content And Storage	4°C, store in dark, DO NOT FREEZE!
Target Species	Human
Host Species	Rat
Conjugate	Alexa Fluor Plus 488
Applications	Flow Cytometry
Form	Liquid
Gene Accession No.	P25963
Isotype	IgG2b κ
Concentration	1 mg/mL
Antigen	IkB alpha
Gene Symbols	NFKBIA
Regulatory Status	RUO
Purification Method	Affinity chromatography
Gene Alias	AI462015; ECI-6; ECI-6/IKBA; I kappa B-alpha; I(Kappa)B(alpha); I79_018146; IkappaB alpha; IkappaBalpha; I-kappaBalpha; I-kappa-B-alpha; Ikba; IKBalpha; IKB-alpha; Inhibitor of nuclear factor of kappa light chain gene enhancer in B-cells alpha; Inhibitor of nuclear factor of kappa light chain gene enhancer in B-cells, alpha; MAD3; MAD-3; Major histocompatibility complex enhancer-binding protein MAD3; NF kappa B inhibitor alpha; NF-kappaB inhibitor alpha; NF-kappa-B inhibitor alpha; NFKB inhibitor alpha; NFKBI; Nfkbia; nuclear factor of kappa light chain gene enhancer in B-cells; nuclear factor of kappa light chain gene enhancer in B-cells inhibitor, alpha; nuclear factor of kappa light polyp gene enhancer in B-cell 1; nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, alpha; nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha; Rel-associated pp40; REL-associated protein pp40; RL/IF-1
Gene	NFKBIA
Product Type	Antibody
Gene ID (Entrez)	4792
Formulation	Proprietary buffer with 0.008% Bromonitrodioxane, 0.008% Methylisothiazolone; pH 6.8
Classification	Recombinant Monoclonal
Primary or Secondary	Primary
Clone	MFRDTRK

357

Invitrogen™ Mouse Adiponectin ELISA Kit

ELISA

Pricing & Availability
Specifications

Shipping Condition	Wet Ice
Protein Family	Cytokines & Receptors
Immunoassay Kit Format	Sandwich ELISA Kit
Target Species	Mouse
Conjugate	HRP
Interassay CV	5.2%
Intraassay CV	2.6%
Substrate Type	Adipokines
Gene Symbol	ADIPOQ
Total Assay Time	3 hr 20 min
Assay Sensitivity	0.05 ng/mL
Storage Requirements	2-8°C
Assay Range	0.125-8 ng/mL
Test Time	1 hr 20 min
Accession Number	Q60994
Regulatory Status	RUO
Target	Adiponectin
Kit Contents	Pre-coated 96 well plate, Standard, Standard Dilution Buffer, HRP conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Standard Dilution Buffer,HRP conjugated Detection Antibody,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
Gene Alias	30 kDa adipocyte complement-related protein; 30kDa; Acdc; Acrp30; Ad; adipo; adipocyte complement related protein; adipocyte complement related protein of 30 kDa; adipocyte complement-related 30 kDa protein; adipocyte complement-related protein of 30 kDa; adipocyte, C1Q and collagen domain containing; adipocyte, C1q and collagen domain-containing protein; Adipocyte-specific protein AdipoQ; adiponectin; adiponectin, C1Q and collagen domain containing; Adipoq; Adipose most abundant gene transcript 1 protein; adipose specific collagen-like factor; ADIPQTL1; ADPIOQ; ADPN; Apm1; APM-1; APN; GBP28; Gelatin-binding protein; gelatin-binding protein 2830 kDa adipocyte complement-related protein; 30kDa; Acdc; Acrp30; Ad; adipo; adipocyte complement related protein; adipocyte complement related protein of 30 kDa; adipocyte complement-related 30 kDa protein; adipocyte complement-related protein of 30 kDa; adipocyte, C1Q and collagen domain containing; adipocyte, C1q and collagen domain-containing protein; Adipocyte-specific protein AdipoQ; adiponectin; adiponectin, C1Q and collagen domain containing; Adipoq; Adipose most abundant gene transcript 1 protein; adipose specific collagen-like factor; ADIPQTL1; ADPIOQ; ADPN; Apm1; APM-1; APN; GBP28; Gelatin-binding protein; gelatin-binding protein 28; 30kDa; APN; Acdc; Acrp30; Ad; GBP28; adipo; apM1
Product Type	ELISA
For Use With (Equipment)	Absorbance Microplate Reader
Gene ID (Entrez)	11450
Sample Type	Plasma,Serum,Supernatant
Label or Dye	HRP

358

Invitrogen™ Human APRIL ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Human
Conjugate	HRP
Interassay CV	7.1%
Intraassay CV	8.1%
Gene Symbol	TNFSF13
Total Assay Time	3 hr 10 min
Sample Volume	Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity	0.4 ng/mL
Storage Requirements	2-8°C
Assay Range	0.78-50.0 ng/mL
For Use With (Application)	ELISA
Test Time	1 hr 20 min
Accession Number	O75888
Regulatory Status	RUO
Target	APRIL
Kit Contents	Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Lysis Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Sample Diluent,Assay Buffer concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Lysis Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Absorbance Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	8741
Sample Type	Plasma,Serum,Supernatant
Label or Dye	Biotin
Synonym	TNF 13, TALL 2, TRDL 1, CD256 antigen

359

Invitrogen™ Human L-Selectin (CD62L) (Soluble) ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Human
Conjugate	HRP
Interassay CV	4.2%
Intraassay CV	3.7%
Gene Symbol	SELL
Total Assay Time	2 hr 10 min
Sample Volume	Plasma 10 μL, Serum 10 μL, Supernatant 10 μL
Assay Sensitivity	0.198 ng/mL
Storage Requirements	2-8°C
Assay Range	0.4-25.0 ng/mL
For Use With (Application)	ELISA
Test Time	1 hr 20 min
Accession Number	P14151
Regulatory Status	RUO
Target	L-Selectin (CD62L)
Kit Contents	Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, HRP-conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Sample Diluent,Assay Buffer concentrate,HRP-conjugated Detection Antibody,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Absorbance Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	6402
Sample Type	Plasma,Serum,Supernatant
Label or Dye	HRP
Synonym	Lymphocyte adhesion molecule 1, CD62L, Leu-8

360

Invitrogen™ Human IL-21 ELISA Kit

ELISA

Pricing & Availability
Specifications

Target Species	Human
Conjugate	HRP
Interassay CV	7.7%
Intraassay CV	5.8%
Gene Symbol	IL21
Total Assay Time	4 hr 10 min
Sample Volume	Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Assay Sensitivity	20.0 pg/mL
Storage Requirements	2-8°C
Assay Range	78-5000 pg/mL
For Use With (Application)	ELISA
Test Time	1 hr 20 min
Accession Number	Q9HBE4
Regulatory Status	RUO
Target	IL-21
Kit Contents	Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Sample Diluent,Assay Buffer concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment)	Absorbance Microplate Reader
Product Type	ELISA
Gene ID (Entrez)	59067
Sample Type	Plasma,Serum,Supernatant
Label or Dye	Biotin
Synonym	Protein 3-10 C, CXCL8, NAP-1, GCP-1

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